This department was established as a separate entity in Panagal building in 1923, and a research laboratory was established thereafter under the guidance and advice of a biochemist, King Wooton, from the UK. The first three year post graduate degree (MD) in Biochemistry was awarded in 1977 and in the following years a separate research laboratory was set up for postgraduate students. A separate 24 hour clinical laboratory was set up in 1998 near the KGH post office. Well established 24 hour clinical laboratory facilities are being provided since 2014 initiated by the then Superintendent of KGH.

Today, the clinical biochemistry laboratory provides clinical support to all of KGH and runs an average of 2500 tests per day and approximately 75,000 biochemical tests per month. The department has been part of the wining thr “AIIMS CEREBRUM” tropy froim AIIMS Mangalagiri yearly